Prediction of Cellular Burden with Host–Circuit Models

Heterologous gene expression draws resources from host cells. These include vital components to sustain growth and replication, the resulting cellular burden is a widely recognized bottleneck in design of robust circuits. In this tutorial we discuss use computational models that integrate circuits physiology Through various cases, illustrate power host–circuit predict impact parameters on both circuit functionality. Our approach relies new generation for microbial can flexibly accommodate resource bottlenecks encountered design. Adoption modeling paradigm facilitate fast cycles synthetic biology.